The efficiency role of African Nightshades in the horizontal transmission of Cucumber mosaic virus and Potyviruses in Kenia (East Africa)

Résumé

Nightshades production is constrained by pests and diseases which severely impacts the quantity and quality available in the value chain. We Hypothesedthat  African Nightshades could be serving as a reservoir for Cucumber mosaic virus (CMV) and Potyviruses in Kenia.Suspicoius African Nightshades leaves were collected to Kenia and propagated in Nicotiana benthamiana, Nicotiana rustica, Nicotiana samsunandgreenhouse grown Nightshade (Solanum  scabrum ). Symptomated leaves for biotest plants and original material were tested by  DAS-ELISA forCMV and by ACP-ELISA for Potyviruses characterization. In order to apply these estimates we must assume that the distribution of the values of replicate data points is normal around the mean of the values. The Standard deviation (SD) was calculed and basing on the calculation of cuf-off (cuf-oof=(mean + 3SD)+10%); the lowest detectable analyte concentration gived a response that had a statistically significant difference from the response of the zero analytic concentration was the detection limit. In 28surveyedfarms, CMV (cuf-oof= 0.24) and Potyviruses(cuf-oof= 0.12) were detected in 20 farms.These results clearly demonstrated the epidemiology of CMV and Potyviruses in the farmlands in Kenyan.